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Fig. 3 | Molecular Medicine

Fig. 3

From: Platelet-rich plasma inhibits Adriamycin-induced inflammation via blocking the NF-κB pathway in articular chondrocytes

Fig. 3

PRP suppresses doxorubicin exposure-induced inflammation. ATDC5 cells were treated with PRP and doxorubicin for 24 h. A Western blot results showing the protein levels of SOX9, Aggrecan, and β-actin. B Real-time PCR to detect mRNA levels of Col2A1, Col10A1, Aggrecan, ALP, MMP-1, MMP-3, and MMP-13. C Western blot to exam the protein levels of inflammatory factors COX-2 and iNOS, and β-actin. D Real-time PCR to detect mRNA levels of TNF-α, IL-6, and IL-1β. At least three independent experiments were performed. *p < 0.05 vs control of the same doxorubicin treatment; #p < 0.05 vs vehicle of the same TGFβ treatment

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