Fig. 1

Intraabdominal sepsis results in a loss of PerC B-1a cells, while CIRP^−/− mice are protected from the loss of B-1a cells after sepsis. A After 20 h of CLP, peritoneal cavity lavage samples were collected. PerC lavage eCIRP concentration was assessed with ELISA. Data are expressed as means ± SE (n = 5–7 mice/group). The groups were compared by Student’s t-test (*p < 0.05 vs. sham mice). WT and CIRP^−/− mice were randomly assigned to sham or CLP groups. 20 h after surgery, PerC lavage samples were collected for analysis. B Representative dot blots of the gating strategy of B-1a cells of sham and CLP mice are shown. C, D Status of B-1a cell’s frequencies (%) and numbers after surgery are shown. Data are expressed as means ± SE (n = 10–14 mice/group). The groups were compared by one-way ANOVA and Student–Newman–Keuls (SNK) method (*p < 0.05 vs. WT sham and ^#p < 0.05 vs. WT CLP mice)