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Fig. 5 | Molecular Medicine

Fig. 5

From: Extracellular CIRP decreases Siglec-G expression on B-1a cells skewing them towards a pro-inflammatory phenotype in sepsis

Fig. 5

An absence of Siglec-G results in greater production of IL-6 by B-1a cells in response to rmCIRP than WT cells. A PerC B-1a cells were collected from WT and Siglec-G−/− mice and stimulated in vitro with rmCIRP or PBS vehicle for 24 h. Supernatant was assessed by ELISA for IL-6 production. Data are expressed as means ± SE (n = 4–8 mice/group) and compared by one-way ANOVA and Student–Newman–Keuls (SNK) method (*p < 0.05 vs. WT PBS treated mice and #p < 0.05 vs. WT rmCIRP treated mice). B Blocking Siglec-G by its neutralizing Abs increases IL-6 production following rmCIRP treatment. PerC B-1a cells were collected from WT mice and pretreated with Siglec-G blocking Ab or IgG control. The cells were stimulated in vitro with rmCIRP or PBS vehicle for 24 h. Supernatant was assessed by ELISA for IL-6 production. Data are expressed as means ± SE (n = 8 mice/group) and compared by one-way ANOVA and Student–Newman–Keuls (SNK) method (*p < 0.05 vs. PBS IgG treated mice and #p < 0.05 vs. rmCIRP IgG treated mice)

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