Fig. 6From: Extracellular CIRP decreases Siglec-G expression on B-1a cells skewing them towards a pro-inflammatory phenotype in sepsisNeutralizing Siglec-G in B-1a cells causes more TNF-α, and IL-6 and less IL-10 production in rmCIRP-treated macrophage co-cultures. PerC macrophages were co-cultured with B-1a cells that had been pretreated with Siglec-G blocking Ab or IgG control and then stimulated with rmCIRP or PBS vehicle for 24 h. Supernatant A TNF-α, B IL-6, and C IL-10 concentration were assessed with ELISA. Data are expressed as means ± SE (n = 8 mice/group) and compared by one-way ANOVA and Student–Newman–Keuls (SNK) method (*p < 0.05 vs. PBS stimulated macrophages, #p < 0.05 vs. macrophages cultured with IgG treated B-1a cells stimulated with rmCIRP, †p < 0.05 vs. untreated B-1a cells stimulated with rmCIRP)Back to article page