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Fig. 4 | Molecular Medicine

Fig. 4

From: CircHYBID regulates hyaluronan metabolism in chondrocytes via hsa-miR-29b-3p/TGF-β1 axis

Fig. 4

CircHYBID expression and hyaluronan (HA) accumulation were decreased by interleukin (IL)-1β treatment. IL-6 (A), TNF-α (B), and HA (E) levels in cell culture supernatant were measured using enzyme-linked immunosorbent assay (ELISA). The mRNA expression of circHYBID (hsa_circ_0003893) (C), HA-binding protein involved in hyaluronan depolymerization (HYBID) (D), HA synthase 1 (HAS1), HA synthase 2 (HAS2), and HA synthase 3(HAS3) (F) were analyzed by quantitative real-time polymerase chain reaction in chondrocytes in the presence or absence of IL-1β treatment. The protein expression of HAS1, HAS2, HAS3, and HYBID (G) was analyzed by Western blotting in chondrocytes in the presence or absence of IL-1β treatment. GAPDH was used as the internal control. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001

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