Fig. 7
From: CircHYBID regulates hyaluronan metabolism in chondrocytes via hsa-miR-29b-3p/TGF-β1 axis
circHYBID acts as a sponge for hsa-miR-29b-3p in OA. A The expression of hsa-miR-29b-3p in IL-1β-induced chondrocytes with or without overexpressed circHYBID was analyzed using quantitative real-time polymerase chain reaction (PCR). B Dual-luciferase reporter assays confirmed the potential binding site of circHYBID and hsa-miR-29b-3p. IL-6 (C), TNF-α (D), and HA (F) levels in cell culture supernatant were measured using enzyme-linked immunosorbent assay in IL-1β-induced chondrocytes with overexpressed circHYBID or miR-29b-3p mimic treatment. The mRNA expressions of HYBID (E) and HAS2 (G) was assessed using quantitative real-time PCR. H The protein expressions of HAS2 and HYBID was detected using Western blotting. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001