Fig. 1
From: Specific detection of high mobility group box 1 degradation product with a novel ELISA
Impact of HMGB1 or desHMGB1 administration on cecal ligation and puncture (CLP)-induced sepsis in mice. A Purity of HMGB1 (2 μg/lane) and desHMGB1 (2 μg/lane) was analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by Coomassie Brilliant Blue (CBB) staining. B Male C57BL/6J mice were subjected to a mild model of CLP-induced sepsis. Mice were randomly divided into the following three groups, each containing 10 animals: CLP alone, CLP with HMGB1 administration, and CLP with desHMGB1 administration. Either 2 mg/kg of HMGB1, 2 mg/kg of desHMGB1, or an equal volume of saline was administered into the tail veins of mice at 6 and 24 h after CLP (the time points are indicated by arrows), and the survival rate was monitored for 7 days. The survival rate of the CLP with HMGB1 group was significantly lower than that of the CLP with desHMGB1 group or the CLP alone group (P < 0.01, log-rank test)