Fig. 3
From: Specific detection of high mobility group box 1 degradation product with a novel ELISA
Specificity of the monoclonal antibody 47C1. A Purified HMGB1 and desHMGB1 were subjected to SDS-PAGE followed by CBB staining (2 μg protein/lane) or western blotting (50 ng protein/lane). The anti-HMGB1 polyclonal antibody (ab 18256) detected both HMGB1 and desHMGB1, while the anti-desHMGB1 monoclonal antibody 47C1 specifically detected desHMGB1. B ELISA for desHMGB1 was constructed as described in “Methods” section. In this assay system, large amounts of intact HMGB1 (320 ng/mL) did not interfere with the detection of desHMGB1 (12.5–50 ng/mL), indicating that the anti-desHMGB1 monoclonal antibody 47C1 did not interact at all with intact HMGB1