Fig. 4

Plasma levels of desHMGB1 in response to rTM treatment in CLP-induced septic mice. Male C57BL/6J mice were subjected to a severe model of CLP-induced sepsis. Mice were randomly divided into the following two groups: those treated with saline and those treated with rTM (5 mg/kg, intravenously) at 6 h after CLP. Plasma samples were collected at 0, 6, 12, and 24 h after CLP, and the plasma levels of desHMGB1 (A) and HMGB1 (B) were analyzed using ELISA. Six mice were allocated to each time point in each group; however, one mouse in each group became moribund before 24 h. These two mice were sacrificed before 24 h and excluded from further analyses. Data are shown as means ± standard deviations. Statistical significance was determined using the Holm–Šidák method. *P < 0.05