Fig. 5

The effect of miR-26a-5p was partially mediated by CDK6. A The putative binding site between miR-26a-5p and CDK6 was predicted by Targetscan. B 293 T cells were transfected with miR-26a-5p mimics or miR-NC, and the mRNA level of miR-26a-5p was detected by qRT-PCR. C 293 T cells were co-transfected with miR-26a-5p mimics or miR-NC and Luc-CDK6-WT or Luc-CDK6-MUT, and the relative luciferase reporter activity was evaluated by dual luciferase reporter system. D and E BV-2 cells were transfected with miR-26a-5p or miR-NC, and the expression of CDK6 was detected by qRT-PCR (D) and western blot (E). F BV-2 cells were treated by OGD/R, and then treated with 200 μg/mL Exo-miR-26a-5p mimics or Exo-miR-NC, and the protein level of CDK6 was evaluated by western blot. G Mice were induced by MCAO/R, and then injected with 200 μL/mice Exo-miR-NC or Exo-miR-26a-5p mimics. The protein level of CDK6 in brain tissues was detected by western blot. *p < 0.05 **p < 0.01