Fig. 1

MiR-125b was downregulated in the heart tissues of HF mice. Mice were treated with or without TAC, and the structure and function of mice hearts were evaluated ten weeks after TAC operation by high resolution small animal ultrasound system and analyzed by M mode. A The mRNA levels of ANP, BNP and β-MHC were evaluated by qRT-PCR (n = 5). B Representative images of echocardiographic measurements of mice hearts (n = 8). C Left ventricular ejection fraction (EF%) (n = 8). D Left ventricular fractional shortening (FS%) (n = 8). E Left ventricular septal thickness; diastole (IVS; d) (n = 8). F Left ventricular posterior wall thickness; diastole, (LVPW; d) (n = 8). G Left ventricular mass/body weight (LV mass/BW) (n = 8). H The mRNA level of miR-125b was evaluated by qRT-PCR (n = 5). I Agomir-125b or agomir-NC was injected into mice, and the mRNA level of miR-125b was evaluated by qRT-PCR (n = 5). ^* p < 0.05, ^** p < 0.01, ^*** p < 0.001 vs. sham group or control group