Fig. 3

MiR-125b overexpression inhibited cardiomyocyte apoptosis in HF mice. Mice were treated with or without TAC. TAC mice were tail intravenously injected with agomir-125b or agomir-NC. A The protein expression of C-caspase 3, caspase 3, Bcl-2 and Bax in heart tissues was detected by Western blot (n = 5). B–D The relative expression changes in C-caspase 3 (B), Bcl-2 (C) and Bax (D) were analyzed by Image Lab™ Software (n = 5). E Representative images of TUNEL staining of heart tissues by confocal microscopy (n = 8). F The apoptosis rate was calculated as the ratio of TUNEL positive nuclei to DAPI-stained nuclei (n = 5). ^* p < 0.05, ^** p < 0.01, ^*** p < 0.001 vs. sham group; ^# p < 0.05, ^## p < 0.01, ^### p < 0.001 vs. TAC group