Fig. 4

BAK1 was a target of miR-125b. A The putative binding sites between miR-125b and 3′ UTR of BAK1 was predicted by Targetscan database. B The relative luciferase activity in HEK293T cells was evaluated by dual luciferase report system (n = 6). ^*** p < 0.001 vs. miR-NC group. C Agomir-125b or agomir-NC was tail intravenously injected into normal mice, and the protein expression of BAK1 was evaluated by Western blot (n = 5). ^** p < 0.01, ^*** p < 0.001 vs. control group. D Agomir-125b or agomir-NC was tail intravenously injected into TAC mice, and the protein expression of BAK1 was evaluated by Western blot (n = 5). ^** p < 0.01, ^*** p < 0.001 vs. sham group. E and F OE-BAK1 or OE-NC was tail intravenously injected into normal mice, then mRNA expression (E) and protein expression (F) was evaluated (n = 5). ^*** p < 0.001 vs. control group