Fig. 1
Propofol decreases the expression of MALAT1 in I/R mouse model and H/R cell model. A MALAT1 with high species conservation in human, rats and mice, as detected through UCSC Genome Browser (http://genome.ucsc.edu/). The gene was amplified with a magnification of 1.5 × . Heights of peak and color depth represent intensity of species conservation. B Measurement on PaO2 levels of mice (8 mice each group; *p < 0.05, compared with that of the sham group; #p < 0.05, compared with the I/R group). C Measurement on W/D ratio of mice (8 mice each group; *p < 0.05, compared with that of the sham group; #p < 0.05, compared with the I/R group). D Pathological changes and lung tissue injury scoring in mice, as detected by H&E staining (8 mice each group; scale bar: 25 μm, *p < 0.05, compared with that of the sham group; #p < 0.05, compared with the I/R group). E Cell apoptosis in lung tissues as determined by TUNEL staining (8 mice each group; scale bar: 25 μm, *p < 0.05, compared with that of the sham group; #p < 0.05, compared with the I/R group). F Morphology of PMVECs and CD14 immunofluorescent images. G Cell viability as assessed by CCK8 assay (*p < 0.05, compared with that of the control group; #p < 0.05, compared with that of the control + Propofol group). H Cell apoptosis as assessed by flow cytometry (*p < 0.05, compared with that of the control group; #p < 0.05, compared with the H/R group). I Expression of MALAT1 in lung tissues of mice as measured by RT-qPCR (8 mice each group; * p < 0.05, compared with that of the sham group; #p < 0.05, compared with the I/R group). J Expression of MALAT1 in cells as measured by RT-qPCR (*p < 0.05, compared with that of the control group; #p < 0.05, compared with the H/R group). Cellular experiments were repeated 3 times independently