Fig. 2

Overexpressing MALAT1 curtails alleviating effects of Propofol. A MALAT1 overexpression efficiency in cells as detected by RT-qPCR (*p < 0.05, compared with the oe-NC group). B MALAT1 overexpression efficiency in mice as detected by RT-qPCR (8 mice in each group; *p < 0.05, compared with that of the sham group; ^#p < 0.05, compared with the I/R + oe-NC group; $ p < 0.05 compared with the I/R + oe-NC + Propofol group). C PaO₂ level in mice (8 mice in each group; *p < 0.05, compared with that of the sham group; ^#p < 0.05, compared with the I/R + oe-NC group; ^$p < 0.05 compared with the I/R + oe-NC + Propofol group). D W/D ratio in lung tissues of mice (8 mice in each group; *p < 0.05, compared with that of the sham group; ^#p < 0.05, compared with the I/R + oe-NC group; ^$p < 0.05 compared with the I/R + oe-NC + Propofol group). E Pathological changes of lung tissues of mice as detected by H&E staining (8 mice each group; *p < 0.05, compared with that of the I/R + oe-NC group; ^#p < 0.05, compared with the I/R + oe-NC + Propofol group). F Cell apoptosis in lung tissues as determined by TUNEL staining (8 mice each group; *p < 0.05, compared with that of the I/R + oe-NC group; ^#p < 0.05, compared with the I/R + oe-NC + Propofol group). G Expression of MALAT1 in cells as measured by RT-qPCR (*p < 0.05, compared with that of the H/R + oe-NC group; ^#p < 0.05, compared with the H/R + oe-NC + Propofol group). H Cell apoptosis as determined by flow cytometry (p < 0.05, compared with that of the H/R + oe-NC group; ^#p < 0.05, compared with the H/R + oe-NC + Propofol group). Cellular experiments were repeated 3 times independently