Fig. 4
Overexpressing GSK3β facilitates autophagy and inflammation in I/R mice. A Expression of GSK3β in lung tissues of mice as determined by immunohistochemistry (8 mice in each group; *p < 0.05, compared with the I/R + oe-NC group; #p < 0.05, compared with the I/R + oe-NC + Propofol group). B PaO2 level of mice (8 mice in each group; *p < 0.05, compared with the sham group; #p < 0.05, compared with the I/R + oe-NC group; $p < 0.05, compared with the I/R + oe-NC + Propofol group). C W/D ratio of mice (8 mice in each group; *p < 0.05, compared with the sham group; #p < 0.05, compared with the I/R + oe-NC group; $p < 0.05, compared with the I/R + oe-NC + Propofol group). D The expressions of GSK3β and autophagy-related protein levels in lung tissues as detected by Western blot analysis (8 mice in each group; *p < 0.05, compared with that of the sham group; #p < 0.05, compared with the I/R + oe-NC group; $p < 0.05, compared with the I/R + oe-NC + Propofol group). E Pathological changes of lung tissues of mice as detected by H&E staining (8 mice each group; *p < 0.05, compared with that of the I/R + oe-NC group; #p < 0.05, compared with the I/R + oe-NC + Propofol group). F Cell apoptosis in lung tissues as determined by TUNEL staining (8 mice each group; *p < 0.05, compared with that of the I/R + oe-NC group; #p < 0.05, compared with the I/R + oe-NC + Propofol group). G Levels of TNF-α, IL-1β, and IL-18 in supernatant of lung tissues of mice as measured by ELISA (8 mice in each group; *p < 0.05, compared with the sham group; #p < 0.05, compared with the I/R + oe-NC group; $p < 0.05, compared with the I/R + oe-NC + Propofol group). The experiment was repeated 3 times independently