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Fig. 4 | Molecular Medicine

Fig. 4

From: 2-O, 3-O desulfated heparin (ODSH) increases bacterial clearance and attenuates lung injury in cystic fibrosis by restoring HMGB1-compromised macrophage function

Fig. 4

Phagocytic function is compromised in macrophages isolated from CF mice. Bone marrow derived macrophages (BMDM) and alveolar macrophages (AM) isolated from C57BL/6 mice (WT, n = 3 mice) and CFTR−/− mice (CF, n = 3 mice) were cultured. Phagocytosis assay was performed on A. AM and B. BMDM by incubating cells with FITC-labeled latex mini-beads (green stain) for 1 h and then staining with DAPI (blue nuclear stain) and phalloidin (red cytoskeletal stain) to visualize the amount of phagocytosed beads per cell. Fluorescent micrographs were quantified and the phagocytic ability of macrophages was represented as a percent phagocytic activity normalized to WT control group. Each value represents mean ± SEM of three independent experiments for each group. *, P < 0.05, ****, P < 0.0001, compared to control groups. C To determine if the magnitude of phagocytosis impairment in CF macrophages is similar between the alveoli and bone marrow macrophages, the phagocytosed beads for AM and BMDM isolated from CF mice were compared to their WT counterparts and expressed as the percent of phagocytosis impairment of the WT macrophages. Each value represents mean ± SEM of three independent experiments for each group. *, P < 0.05 compared to AM group. Unpaired t-test was used for comparison between two groups

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