Fig. 5

ODSH decreases airway HMGB1 accumulation in PA infected CF mice. A C57BL/6 mice (WT) and CFTR^−/− mice (CF) were randomized and inoculated with normal saline (without PA) or PA (1 × 10⁷ CFUs/mouse) for 18 h. Bronchoalveolar lavage fluid (BALF) was collected from mice was analyzed for the levels of extracellular HMGB1 by Immunoblot blot analyses. Representative images of immunoreactive bands of HMGB1 in the samples were shown on the top of the bar graphs. B C57BL/6 mice (WT) and CFTR^−/− mice (CF) were intratracheally inoculated with Pseudomonas aeruginosa (PA) at T = 0 and were randomized to receive via i.p. administration of saline or ODSH at 8.3 or 25 mg/kg ta T = 0 and 12 h. BALF was collected 18 h after infection (T = 18 h) and was analyzed for the levels of airway HMGB1 by Western blot analysis. Levels of HMGB1 were represented by relative densitometric values and expressed in AU. Each value represents mean ± SEM of three independent experiments for each group. *, P < 0.05; **, P < 0.01 compared to WT groups infected with PA (A). *, P < 0.05 compared to CF control groups receiving normal saline. One-way ANOVA was used for multiple group comparison to determine statistical significance