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Fig. 3 | Molecular Medicine

Fig. 3

From: Up-regulation of microRNA-135 or silencing of PCSK6 attenuates inflammatory response in preeclampsia by restricting NLRP3 inflammasome

Fig. 3

Up-regulating miR-135 alleviates inflammatory response in H/R-treated trophoblasts. A Detection of miR-135, PCSK5 and NLRP3 expression in H/R-treated HTR-8/SVneo and HPT-8 trophoblasts by qPCR; B Detection of PCSK5 and NLRP3 protein expression in H/R-treated HTR-8/SVneo and HPT-8 trophoblasts by Western blot; C Detection of cell proliferation in H/R-treated HTR-8/SVneo and HPT-8 trophoblasts by colony formation assay; D Detection of cell viability in H/R-treated HTR-8/SVneo and HPT-8 trophoblasts by MTT assay; E Detection of cell apoptosis in H/R-treated HTR-8/SVneo and HPT-8 trophoblasts by flow cytometry; F Detection of Bcl-2 and Bax mRNA expression in H/R-treated HTR-8/SVneo and HPT-8 trophoblasts by qPCR; G Detection of invasion and migration in H/R-treated HTR-8/SVneo and HPT-8 trophoblasts by Transwell test; H Detection of IL-1β and TNF-α expression in H/R-treated HTR-8/SVneo and HPT-8 trophoblasts by ELISA; N = 3, *P < 0.05 vs. the H/R + mimic NC group

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Molecular Medicine

ISSN: 1528-3658