Fig. 2
Hypoxia-induced Pref-1 expression in WI-38 cells. A 90% confluency WI-38 cells were incubated with 1% O2 for 0–2 h. The levels of Pref-1 messenger RNA were detected by using qPCR. Data are presented as the mean ± S.E.M. of three experiments. B 90% confluency WI-38 cells were incubated with different oxygen concentrations for 4 h, and then, Pref-1 and α-tubulin were determined through immunoblots. These are presented as the mean ± S.E.M. (n = 3). *P < 0.05, compared with control (at O2 21%). C 60% confluency WI-38 cells were exposed with 1% O2 for 4 h. HIF-1α, Pref-1, and α-tubulin were detected through immunoblots. These are presented as the mean ± S.E.M. (n = 4). *P < 0.05, compared with control (at O2 21%)