Fig. 4
Involvement of ERK in hypoxia induces a Pref-1 expression in WI-38 cells. A WI-38 cells were pretreated with U0126 for 30 min and then incubated with the hypoxia (1% O2) for 4 h. Levels of Pref-1 and α-tubulin in cell lysates were determined. Data are presented as the mean ± S.E.M. of three experiments. * P < 0.05, compared with the hypoxia-exposed group. Cells were pretreated with U0126 for 30 min and then subjected to hypoxia (1% O2) for 30 min. B PEA3 was immunoprecipitated using anti-PEA3 antibody, and PEA3-p-serine was detected using anti-p-serine antibody. The quantified results were adjusted with PEA3 and expressed as a percentage of control. C Levels of phospho-c-Jun Ser63 and c-Jun were detected using Western blotting in cell lysates. Values represent the means ± S.E.M. of three experiments. * P < 0.05 compared with the hypoxia-exposed group