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Fig. 7 | Molecular Medicine

Fig. 7

From: The long noncoding RNA MEG3 regulates Ras-MAPK pathway through RASA1 in trophoblast and is associated with unexplained recurrent spontaneous abortion

Fig. 7

MEG3 modulated the RAS-MAPK pathway by regulating the expression of the RASA1 gene. Western blot assay: a Effects of pcDNA-RASA1 and si-RASA1 on the RASA1 protein level in PT-U cells. b RASA1-mediated regulation in the activities of Raf, MEK, and ERK proteins in the RAS-MAPK pathway by MEG3. c The CCK-8 assay confirmed that RASA1 mediates the regulation of the proliferative capacity of PT-U cells by MEG3. d Transwell assay confirmed that RASA1 mediates the regulation of the invasive capacity of PT-U cells by MEG3. e and f CCK-8 and flow cytometry assay show that MEG3 has no effect when there is low RASA1 in PT-U cells (Vector + si-RASA1 V.S. MEG3 + si-RASA1). *P < 0.05

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