Fig. 1
Upregulated expression of miR-182-5p in breast cancer tissues and cells. A Venn diagram of the identified differential miRNAs in breast cancer-related datasets GSE26659, GSE35412-1, GSE35412-2, GSE45666 and GSE58027; B A heat map of 8 intersected miRNA expression in breast cancer-related datasets GSE26659, GSE35412-1, GSE35412-2, GSE45666 and GSE58027 (the right shows the color scale of log2FC value); C Expression of 8 intersected miRNAs (miR-183, miR-454, miR-425, miR-145, miR-130b, miR-99a, miR-143, miR-182-5p) in MDA-MB-231 cells detected by RT-qPCR, the statistical power was 1; D A box plot of miR-182-5p expression in breast cancer tissue and adjacent normal tissue in the dataset GSE45666 (the left blue box represents the expression of adjacent normal tissue samples, and the right red box represents the expression of breast cancer tissue samples); E Expression of miR-182-5p in cancer tissues and adjacent normal tissues of 40 breast cancer patients detected by RT-qPCR, the statistical power was 1; F Expression of miR-182-5p in breast cancer tissues and adjacent normal tissues detected by ISH, the statistical power was 1; G Expression of miR-182-5p in metastasis tissues of 16 breast cancer patients with distant metastasis and cancer tissue of 24 breast cancer patients without distant metastasis detected by ISH, the statistical power was 1; H Expression of miR-182-5p in metastasis tissues of 16 breast cancer patients with distant metastasis and cancer tissue of 24 breast cancer patients without distant metastasis detected by RT-qPCR, the statistical power was 1; I Correlation between the expression of miR-182-5p and the prognosis of 40 breast cancer patients analyzed by Kaplan–Meier curve analysis; J Expression of miR-182-5p in 4 cell lines (MCF-10A, MCF-7, KPL-4 and MDA-MB-231) detected by RT-qPCR, the statistical power was 1. *p < 0.05, ***p < 0.001, ****p < 0.0001 compared with adjacent normal tissues, breast cancer patients without distant metastasis or MCF-10A cell line. The experiment was conducted three times independently