Fig. 3From: Breast cancer cell-derived extracellular vesicles transfer miR-182-5p and promote breast carcinogenesis via the CMTM7/EGFR/AKT axisEVs derived from breast cancer cells transfer miR-182-5p to HUVECs. A Morphological characteristics of EVs derived from the serum of breast cancer patients and MDA-MB-231 cells under a TEM; B Expression of EV marker proteins CD63, Hsp70, TSG101 and Calnexin detected by Western blot analysis; C Diameter of EVs detected by Nanosight Nanoparticle Tracking Analyzer; D Uptake of EVs in HUVECs under a confocal microscope, blue: Hoechst staining, green: PKH67-labeled EVs; E miR-182-5p transfer by EVs to HUVECs detected by immunofluorescence (green fluorescent labeled miR-182-5p, red fluorescent labeled EVs, blue fluorescent represented the nucleus). The experiment was conducted three times independentlyBack to article page