Fig. 2

High-glucose treatment mediated EndMT via the upregulation of PFN2 expression in HUVECs. A Results from the Western blot analysis of CD31, vimentin, αSMA, S100A4 and PFN2 levels in HUVECs with the corresponding treatment. B Compared with the control group, the mRNA expression of CD31 was decreased in hyperglycemic HUVECs. C Compared with the control group, the mRNA expression of vimentin was increased in hyperglycemic HUVECs. D Compared with the control group, the mRNA expression of αSMA was increased in hyperglycemic HUVECs. E Compared with the control group, the mRNA expression of S100A4 was increased in hyperglycemic HUVECs. F Compared with the control group, the mRNA expression of PFN2 was increased in hyperglycemic HUVECs. G Results from the Western blot analysis of PFN2, CD31, vimentin, αSMA and S100A4 levels in HUVECs with the corresponding treatment. H The effects of si-PFN2 were confirmed by qPCR. I Compared with high-glucose treatment, si-PFN2 increased CD31 mRNA expression in hyperglycemic HUVECs. J Compared with high-glucose treatment, si-PFN2 decreased vimentin mRNA expression in hyperglycemic HUVECs. K Compared with high-glucose treatment, si-PFN2 decreased αSMA mRNA expression in hyperglycemic HUVECs. L Compared with high-glucose treatment, si-PFN2 decreased S100A4 mRNA expression in hyperglycemic HUVECs. (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, n = 5/group)