Fig. 1

MiR-181a-5p is expressed at low levels in FD BMSCs. a MicroRNA sequencing analysis showed the differentially expressed miRNAs between FD BMSCs and BMSCs. b Quantitative real-time PCR analysis was used to detect miR-181a-5p, miR-145-3p, miR-98-3p and miR-92b-5p expression between FD BMSCs and BMSCs. c cAMP levels in BMSCs treated with cAMP or IBMX were analyzed by ELISA. MiR-181a-5p, miR-145-3p, miR-98-3p and miR-92b-5p expression in BMSCs treated with cAMP (d) or IBMX (e) was analyzed by quantitative real-time PCR. f Quantitative real-time PCR analysis was used to detect miR-145-3p, miR-98-3p and miR-92b-5p expression in FD BMSCs transfected with miR-181a-5p mimics. g Quantitative real-time PCR analysis was used to detect miR-145-3p, miR-98-3p and miR-92b-5p expression in BMSCs transfected with miR-181a-5p inhibitor. h Venn diagram showing the number of miR-181a-5p target genes predicted by performing miRDB, miRPathDB, miRWalk, and TargetScan algorithms. i KEGG analysis of the target genes of miR-181a-5p. The data are presented as the mean ± S.E.M. values (n ≥ 3). ^#P > 0.05; *P < 0.05; ***P < 0.001