Fig. 4

Low miR-181a-5p expression promotes FD BMSC-induced osteoclast differentiation and formation. a BMSC- and FD BMSC-induced osteoclast differentiation was analyzed by TRAP staining. b Representative image of the F-Actin ring structure in osteoclasts. Osteoclast differentiation-related gene expression was detected by quantitative real-time PCR (c) and western blot (d). e Effect of miR-181a-5p mimics on FD BMSC-induced osteoclast differentiation was analyzed by TRAP staining. f Representative image of the F-Actin ring structure in osteoclasts under miR-181a-5p mimics treatment. Osteoclast differentiation-related gene expression was detected by quantitative real-time PCR (g) and western blot (h) in osteoclasts induced by FD BMSCs transfected with miR-181a-5p mimics. i Effect of the miR-181a-5p inhibitor on BMSC-induced osteoclast differentiation was analyzed by TRAP staining. j Representative image of the F-Actin ring structure in osteoclasts under miR-181a-5p mimics treatment. Osteoclast differentiation-related gene expression was detected by quantitative real-time PCR (k) and western blot analysis (l) in osteoclasts induced by BMSCs transfected with miR-181a-5p inhibitor. Scale bar, 100 μm. The data are presented as the mean ± S.E.M. values (n = 3). ^#P > 0.05; *P < 0.05; **P < 0.01; ***P < 0.001