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Fig. 5 | Molecular Medicine

Fig. 5

From: The antioxidant N-acetylcysteine promotes immune response and inhibits epithelial-mesenchymal transition to alleviate pulmonary fibrosis in chronic obstructive pulmonary disease by suppressing the VWF/p38 MAPK axis

Fig. 5

NAC affects EMT via the VWF/p38 MAPK axis. A Efficiency of sh-p38 MAPK-1 and sh-p38 MAPK-2 detected by RT-qPCR. B Efficiency of sh-p38 MAPK-1 and sh-p38 MAPK-2 detected by Western blot analysis. CSE cells were transduced with NAC and oe-VWF and/or sh-p38 MAPK. C Expression of VWF and p38 MAPK, and the extent of p38 MAPK phosphorylation in CSE cells determined by Western blot analysis. D CSE cell migration detected by Transwell assay. E CSE cell invasion detected by Transwell assay. F Protein levels of E-cadherin and N-cadherin in CSE cells determined by Western blot analysis. ***p < 0.001. ****p < 0.0001. Data are shown as the mean ± standard deviation of three technical replicates. Data among multiple groups were compared by one-way ANOVA with Tukey’s post hoc test

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Molecular Medicine

ISSN: 1528-3658