Fig. 1
From: Placental 13C-DHA metabolism and relationship with maternal BMI, glycemia and birthweight
Placental lipids containing 12C-DHA and 13C-DHA in placental explants from 17 placenta incubated for 48 h with 24 µmol/L 13C-DHA at a physiological glucose concentration (5 mmol/L). A Amount of each lipid (pmol/mg) with percentages showing relative amount of each lipid class compared to total 12C-DHA or 13C-DHA lipids. Brackets show standard deviation. Colours represent individual lipids. B Boxplots showing the enrichment of each lipid with 13C-DHA. Enrichment is the percentage of each lipid containing 13C-DHA calculated by the formula 100 * 13C-lipid/(13C-lipid + 12C-lipid). DHA docosahexaenoic acid, DG diacylglycerol, LPC lyso-phosphatidylcholine, LPE lyso-phosphatidylethanolamine, PC phosphatidylcholine, PE-P phosphatidylethanolamine-plasmalogen, TG triacylglycerols. Labeled TGs contained 13C-DHA and two other fatty acids which cannot be identified with certainty. Since the total number of fatty acid carbons (x) and the total number of fatty acid double bonds (y) is known for each TG (format x:y), the combined carbon count and saturation of the two remaining fatty acids can be calculated, then predicted based on known abundance in humans. Two TGs contained 13C-DHA and two saturated lipids (TG 54:6 [predicted 16:0_16:0_22:6], TG 56:6 [predicted 16:0_18:0_22:6]), two contained DHA with one saturated fatty acid and one mono-unsaturated fatty acid (TG 54:7 [predicted 16:0_16:1_22:6], TG 56:7 [predicted 16:0_18:1_22:6 or 16:1_18:0_22:6]) and five TGs contained DHA with unknown combinations of various unsaturated lipids (TG 56:8, TG 58:8, TG 56:9, TG 58:9 and TG 58:10)