Fig. 4

DKFZp434J0226 (DKFZ) promotes PDAC cell proliferation and migration. A, B Verification of DKFZ knockdown (A) and DKFZ overexpression (B) efficiency in MIAPaCa-2 and AsPC-1 cells (n = 3). Data are shown as mean ± SD; *P < 0.05, **P < 0.01. C Cell growth curves of lipofectamine-treated MIAPaCa-2 (Mock) cells, negative siRNA-transfected MIAPaCa-2 (NC) cells, and two DKFZ siRNA-transfected MIAPaCa-2 (si1 and si2; n = 4). Data are shown as mean ± SD; *P < 0.05 vs. Mock and NC; **P < 0.01 vs. Mock and NC. D Cell growth curves of control lentivirus-infected AsPC-1 cells (control) and DKFZ lentivirus-infected AsPC-1 cells (DKFZ) (n = 4). Data are shown as mean ± SD; *P < 0.05 vs. Mock and NC; **P < 0.01 vs. Mock and NC. E Soft agar colony formation assay and relative levels of colonies in Mock, NC, si1, and si2 cells (n = 4). F Soft agar colony formation assay and relative levels of colonies in control and DKFZ cell (n = 4). G Western blotting analysis of PCNA expression in Mock, NC, si1 and si2 MIAPaCa-2 cells. Western blotting analysis of PCNA expression in control and overexpression AsPc-1 cells. H–J Wound healing (H, I) and migration (J) assays using the referred PDAC cells. Quantification using ImageJ (n = 4). E–J Data are shown as mean ± SD; *P < 0.05, **P < 0.01. Scale bars refer to 100 μm