Fig. 6

DKFZp434J0226 (DKFZ) increases the nuclear localization of SF3B6. A Western analysis and quantification of nuclear and cytoplasmic SF3B6 in control and DKFZ-overexpressed AsPC-1 cells. Nuclear and cytoplasmic subcellular fractions were extracted as described in “Materials and methods” section. B Protein levels of SF3B6 in the nucleus and cytoplasm observed using confocal microscope. C DKFZ regulates alternative splicing of SF3B6 downstream MDM4. The alternative spliced transcripts (MDM4-FL, MDM4 full-length transcript; MDM4-S, MDM4 S transcript) in control and DKFZ-overexpressed AsPC-1 cells were verified by RT-qPCR. The percent spliced in index (PSI) was quantified for the alternative splicing events (n = 5). A, C Data are shown as mean ± SD; *P < 0.05, **P < 0.01. Scale bars refer to 20 μm