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Fig. 6 | Molecular Medicine

Fig. 6

From: Gastrin exerts a protective effect against myocardial infarction via promoting angiogenesis

Fig. 6

CCK2R role in the protective effects of gastrin against myocardial infarction. Endothelial cells were treated with gastrin (100 nM) with or without the CCK2R inhibitor, CI988 (100 nM). a Western blot was used to detect the protein levels of vascular endothelial growth factor (VEGFA). Results are expressed as the ratio of VEGFA to GAPDH. n = 4. *P < 0.05, vs. Control; #P < 0.05, vs. gastrin-treated group. b Representative images and quantification of endothelial cell tube formation assay in vitro. Scale bar = 50 µm. n = 4. *P < 0.05, vs. Control; #P < 0.05, vs gastrin-treated group. Myocardial infarction mice were infused with gastrin or/and CI988. c1 Immunofluorescence staining with anti-cluster of differentiation 31 (CD31) antibody showed the microvessel densities in the infarcted border zone 14 days after MI. DAPI is blue, and CD31 is red. Scale bar = 50 µm. c2 Western blot was used to detect the protein levels of CD31 in the peri-infarct area of the heart 14 days after MI. Results are expressed as the ratio of CD31 to GAPDH. n = 8. *P < 0.05, vs. MI group; #P < 0.05, vs gastrin-treated MI mice. d1 Representative M-mode images of hearts with MI 28 days after gastrin or/and CI988 infusion. d2–3 Left ventricle ejection fraction (EF), fractional shortening (FS) 7, 14, and 28 days after MI. n = 8. *P < 0.05, vs. MI + Gastrin + CI988

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