Table 2 Primes sequences used in this study
Gene | Sequences (5′-3′) |
|---|---|
METTL3-forward | CTCCACGCCAGATGCTCC |
METTL3-reverse | GACAGTCCCTGCTACCTCCC |
METTL14-forward | GGTTCTGGGGAGGGGTTG |
METTL14-reverse | ATGAGGCAGTGTTCCTTTGTTC |
WTAP-forward | GCACGCAGGGAAAACATCC |
WTAP-reverse | ATAGTCCGACGCCATCAGG |
NLRP3-forward | TTCGGAGATTGTGGTTGGG |
NLRP3-reverse | TCAGGGAATGGCTGGTGC |
IGF2BP1-forward | TCCCCGATGAGCAGATAGC |
IGF2BP1-reverse | TTTGTGATGTTGCGGATGG |
IGF2BP2-forward | TGGGAGGTGTTGGATGGAC |
IGF2BP2-reverse | TCAAACTGATGCCCGCTTAG |
IGF2BP3-forward | TCCCTACCCGCAGTTTGA |
IGF2BP3-reverse | CGAGAAAGCTGCTTGATGTG |
GAPDH-forward | AATCCCATCACCATCTTC |
GAPDH-reverse | AGGCTGTTGTCATACTTC |
miR-100-5p-forward | GCGAACCCGTAGATCCGAA |
miR-100-5p-reverse | AGTGCAGGGTCCGAGGTATT |
miR-21-5p-forward | GCGCGTAGCTTATCAGACTGA |
miR-21-5p-reverse | AGTGCAGGGTCCGAGGTATT |
miR-221-3p-forward | CGCGAGCTACATTGTCTGCTG |
miR-221-3p-reverse | AGTGCAGGGTCCGAGGTATT |
miR-26a-5p-forward | CGCGTTCAAGTAATCCAGGA |
miR-26a-5p-reverse | AGTGCAGGGTCCGAGGTATT |
miR-143-3p-forward | CGCGTGAGATGAAGCACTG |
miR-143-3p-reverse | AGTGCAGGGTCCGAGGTATT |
miR-27b-3p-forward | GCGCGTTCACAGTGGCTAAG |
miR-27b-3p-reverse | AGTGCAGGGTCCGAGGTATT |
U6-forward | CTCGCTTCGGCAGCACA |
U6-reverse | AACGCTTCACGAATTTGCGT |