Fig. 5

GPER regulates actin polymerization via YAP-ARHGAP29-RhoA-LIMK-Cofilin Signaling. a Protein expression of YAP in chondrocyte treated with G-1 or mechanical stress (n = 3), *P < 0.05, compared with control group. **P < 0.01, compared with control group. ^#P < 0.05, compared with mechanical stress group. ^##P < 0.01, compared with mechanical stress group. b Immunofluorescence staining YAP (n = 3). (c) Cell viabilities for chondrocytes with YAP or scrambled siRNA after treatment with mechanical stress, and G-1 (n = 3), *P < 0.05, compared with DMSO group. **P < 0.01, compared with DMSO group. ^#P < 0.05, compared with mechanical stress + DMSO group. ^##P < 0.01, compared with mechanical stress + DMSO group. d Protein expression of Bax and Bcl-2 for chondrocytes with YAP or scrambled siRNA after treatment with mechanical stress, and G-1 (n = 3), *P < 0.05, compared with DMSO group. **P < 0.01, compared with DMSO group. ^#P < 0.05, compared with mechanical stress + DMSO group. ^##P < 0.01, compared with mechanical stress + DMSO group. e Immunofluorescence staining F-actin and Piezo1 (n = 3). f Protein expression of ARHGAP29-RhoA-LIMK-Cofilin Signaling in chondrocyte treated with G-1 or mechanical stress (n = 3) *P < 0.05, compared with control group. **P < 0.01, compared with control group. ^#P < 0.05, compared with mechanical stress group. ^##P < 0.01, compared with mechanical stress group. All data are presented in mean ± SD