Fig. 1

PLN modified mRNAs overview and mRNA in vitro stability assay. A Schematic representation of PLN protein monomeric structure and of its regulatory domain Ia, Ib and transmembrane domain II, location of regulatory phosphorylated amino acid (pink) and location of L39X mutation (red). B Schematic representation of the different modified mRNA constructs. 5’UTR and analogue cap (green), PLN CDS (dark blue), 3’UTR and Poly-A trail (pink), HA tag (orange), T2A sequence (yellow), mCherry (red), G-> T mutation (red star), black arrows indicate FWD and REV cDNA PCR Primers. C PCR amplified cDNA gel electrophoresis showing the presence of modHA-PLN (1), modHA-L39X (2), modHA-PLN-T2A-mCherry (3), modHA-L39-T2A-mCherry (4) transfected modified mRNA after 4 h, 24 h, and 48 h respectively. D Sanger sequencing of PCR amplified cDNA showing the expected wild-type and mutated sequences of the transfected modRNA constructs