Skip to main content
Fig. 6 | Molecular Medicine

Fig. 6

From: MiR-100 overexpression attenuates high fat diet induced weight gain, liver steatosis, hypertriglyceridemia and development of metabolic syndrome in mice

Fig. 6

MiR-100 overexpression attenuates lipid accumulation in primary cultured hepatocytes. a The illustration shows the potential binding site of miR-100 in the 3′UTR of CD36. b The luciferase activity was reduced when the CD36 3′UTR plasmid and miR-100 precurser molecules (premiR-100) were overexpressed (n = 8). c The luciferase activity was induced when the CD36 3’UTR plasmid was overexpressed together with the miR-100 inhibitory molecule antimiR-100 (n = 8). d Exemplary fluorescence images of BODIPY FL C16 uptake in primary cultured mouse hepatocytes isolated from wildtype and miR-100 overexpressing mice are shown (Magnification: ×400). The hepatocytes were treated with DMSO control or BODIPY FL C16 at 200 nM for 1 h. e The quantification of BODIPY FL C16 revealed a reduced uptake into hepatocytes isolated from miR-100 overexpressing mice. (n = 6–7) Data represent mean values with SEM. *P < 0.05, **P < 0.01 and ***P < 0.001 vs. the corresponding control

Back to article page