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Fig. 5 | Molecular Medicine

Fig. 5

From: Increased hemoglobin and heme in MALDI-TOF MS analysis induce ferroptosis and promote degeneration of herniated human nucleus pulposus

Fig. 5

High levels of Hb and heme induce ferroptosis in herniated tissues. a Hb polymers in herniated nucleus pulposus were detected by MALDI-TOF MS. b The protein levels of CD31, HO-1, Hb α, and Hb β in 5 samples of herniated nucleus pulposus (Pfirrmann scores of 3–4) and 3 samples of nonherniated nucleus pulposus. c Iron levels in 3 samples of herniated nucleus pulposus (Pfirrmann scores of 3–4) and 3 samples of nonherniated nucleus pulposus. d Prussian blue staining of herniated nucleus pulposus (Pfirrmann grade 3–4) and nonherniated nucleus pulposus. e HO-1 and GPX4 protein levels in herniated nucleus pulposus (Pfirrmann grade 3–4) and nonherniated nucleus pulposus, as determined by immunohistochemical analysis. Representative IHC images were taken at a magnification of 200× . f Identification of ROS and ferroptosis-related metabolites in herniated nucleus pulposus (Pfirrmann grade 3–4) and nonherniated nucleus pulposus by high-resolution MALDI-TOF MS

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Molecular Medicine

ISSN: 1528-3658