Fig. 7From: Increased hemoglobin and heme in MALDI-TOF MS analysis induce ferroptosis and promote degeneration of herniated human nucleus pulposusChanges in the Notch signaling pathway in HNPCs after heme treatment. a Notch-related mRNAs in HNPCs were measured by qRT-PCR after 20 μg/mL heme treatment for 24 h with or without 30 μg/mL DFO pretreatment for 1 h. Data were analyzed with GraphPad Prism 5.02 software. *P < 0.05, **P < 0.01. b Notch-related proteins in HNPCs were measured by western blot analysis after 20 μg/mL heme treatment for 24 h with or without 30 μg/mL DFO pretreatment for 1 hBack to article page