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Fig. 2 | Molecular Medicine

Fig. 2

From: Suppression of CRLF1 promotes the chondrogenic differentiation of bone marrow-derived mesenchymal stem and protects cartilage tissue from damage in osteoarthritis via activation of miR-320

Fig. 2

Expression levels of CRLF1 during chondrogenic differentiation of BMSCs. A Flow cytometric analysis of BMSC surface markers. B The deposition of cartilage matrix proteoglycans was determined by Alcian blue staining, nuclei were counterstained by nuclear fast red (scale bar, 100 μm). C Expression of Sox9 and CRLF1 was examined by WB. D Immunofluorescence staining for CRLF1 (green), nuclear stain DAPI (blue), and Merge for green and blue (scale bar, 100 μm); Data are expressed as mean ± SEM and *P < 0.05, **P < 0.01, ***P < 0.01

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