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Fig. 3 | Molecular Medicine

Fig. 3

From: Suppression of CRLF1 promotes the chondrogenic differentiation of bone marrow-derived mesenchymal stem and protects cartilage tissue from damage in osteoarthritis via activation of miR-320

Fig. 3

Overexpression of CRLF1 inhibited BMSCs viability, induced apoptosis and suppressed cell differentiation. A Infection efficiency was determined by microscopic examination of the GFP-expressing cells (scale bar, 100 μm). B The CRLF1 expression in different transfected BMSCs. C The BMSCs viability of different transfected groups. D Flow cytometric analysis of BMSCs apoptosis of different transfected groups. E The expression of apoptosis-related proteins in different transfected groups. F The deposition of cartilage matrix proteoglycans was determined by Alcian blue staining (scale bar, 50 μm). G IHC results of Col2a1 expression in different transfected groups (scale bar, 50 μm). H qPCR showed the relative quantification of ACAN, Sox9, Col2a1, collagen X and MMP13 mRNA levels in each group. I Protein expressions of ACAN, Sox9, Col2a1, collagen X and MMP13were evaluated by western blot. Data are expressed as mean ± SEM and *P < 0.05, **P < 0.01, ***P < 0.01

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Molecular Medicine

ISSN: 1528-3658