Fig. 2
From: MicroRNA regulation of the proliferation and apoptosis of Leydig cells in diabetes
Bioinformatics analysis of testicular miRNA by RNA sequencing. Volcano plot analysis of differentially-expressed miRNAs (A) and mRNAs (B) in the diabetic vs. normal testis from ND and DM rats. The log2 transformation of the fold change in the expression of miRNAs and mRNAs between diabetic and normal testes from each group is plotted on the x-axis. The log p-value (base 10) is placed on the y-axis. Differentially-expressed miRNAs and mRNAs (fold change ≥ 1) are indicated in red (upregulated miRNAs and mRNA in diabetic testis) and green (downregulated miRNAs and mRNA in diabetic testis). Upregulated (miRNA_up_target) and downregulated (miRNA_down_target) miRNA-target genes were predicted online using TargetScan (http://www.targetscan.org/). The overlapping target genes and downregulated (mRNA_lo) or upregulated (mRNA_up, C) mRNAs were identified via Venn diagrams. The miRNA–mRNA regulation networks were constructed using the Gephi software (D). Red dots represent upregulated miRNAs, whereas green dots indicate downregulated miRNAs, and blue dots indicate downstream target genes. KEGG analysis of upregulated and downregulated mRNAs in the miRNA–mRNA regulation networks (E)