Fig. 2

Influence of circ_0007059 overexpression on the cell viability of IFN-induced RMC and HEK293 cells. RMC and HEK293 cells were transfected with a circ_0007059-overexpressing vector or NC vector for 24 h, followed by treatment with 1,000 units/mL IFN for 24 h. A, B Expression level of circ_0007059 in RMC and HEK293 cells was detected by RT-qPCR (biological replicates, 3; technical replicates, 2; repeat time, 3). C, D A CCK-8 assay was used to measure the cell proliferation rate at 24, 48, and 72 h after transfection (biological replicates, 1; technical replicates, 3; repeat time 3). E, F An MTT assay was used to determine cell viability, whereas untreated cells served as controls (biological replicates, 1; technical replicates, 3; repeat time, 3). Results are expressed as the mean ± SEM for technical replicates. *P < 0.05, **P < 0.01 vs. the indicated group