Fig. 5

circ_0007059 targeted miR-1278 and miR-1278, in turn, targeted SHP-1. A Graphical illustration of the conserved circ_0007059 binding motifs in the 3′-UTR of SHP-1 for miR-1278 and miR-1278-binding motifs. B, C a luciferase reporter assay was used to measure luciferase activity, including wild-type or mutated (MU) copies of human circ_0007059 and SHP-1 after transfection an miR-1278 mimic in HEK293 cells. Luciferase activity was normalized to that of Renilla luciferase (biological replicates, 1; technical replicates, 3; repeat time, 3). D, E the expression of miR-1278 and SHP-1 mRNA in renal biopsy samples from patients with LN (n = 30) and adjacent normal tissue samples (n = 10) as measured by RT-qPCR are shown. RMC and HEK293 cells were transfected with a circ_0007059-overexpressing vector or NC vector for 24 h, followed by treatment with 1000 units/mL IFN for 24 h (biological replicates, as indicated; technical replicates, 3; repeat time, 3). F, G miR-1278 levels in RMCs and HEK293 cells were measured by RT-qPCR (biological replicates, 1; technical replicates, 3; repeat time, 3). H, I RT-qPCR and J, K WB was performed to detect SHP-1, STAT3, and phosphorylated STAT3 levels in RMCs and HEK293 cells (biological replicates, 1; technical replicates, 3; repeat time, 3). Results are expressed as the mean ± SEM for technical replicates. *P < 0.05, **P < 0.01 vs. the indicated group