Fig. 6

Effect of an miR-1279 mimic on circ_0007059-induced cell viability, apoptosis, and IFN-associated inflammation of RMCs and HEK293 cells. RMC and HEK293 cells were cotransfected with a circ_0007059-overexpressing vector and/or NC/miR-1278 mimic for 24 h, followed by treatment with 1000 units/mL IFN for 24 h. Expression of A, B miR-1278 or C, D SHP-1 was evaluated by RT-qPCR in cells with different transfected constructs (biological replicates, 1; technical replicates, 3; repeat time, 3). E, F WB was performed to detect SHP-1, STAT3, and phosphorylated STAT3 levels in RMCs and HEK293 cells (biological replicates, 1; technical replicates, 3; repeat time, 3). G, H cell viability of RMCs and HEK293 cells was examined using the MTT assay (biological replicates, 1; technical replicates, 3; repeat time, 3). I, J the number of apoptotic cells was assessed by flow cytometry (biological replicates, 1; technical replicates, 3; repeat time, 3). K, L expression of CXCL10, IFIT1, ISG15, and MX1 mRNA in RMCs and HEK293 cells was determined by RT-qPCR (biological replicates, 1; technical replicates, 3; repeat time, 3). Results are expressed as the mean ± SEM for technical replicates. *P < 0.05, **P < 0.01 vs. the indicated group