Fig. 5

PlGF is identified as a target of miR-214. A The predicted binding sites of miR-214 and PlGF. B Dual luciferase reporter assay analysis of the binding of miR-214 to PlGF. C RT-qPCR determination of the mRNA level of PlGF following miR-214 overexpression in cells. D Western blot analysis of PlGF following miR-214 overexpression in cells. E RIP detection of the binding percentage of miR-214 and PlGF to Ago2, normalized to IgG binding. Data are summarized as mean ± standard deviation. *p < 0.05 vs. anti-IgG. ^#p < 0.05 vs. cells transfected with miR-214 NC. An independent sample t-test was used for comparisons between two groups. Multiple group comparisons were performed using one-way ANOVA, followed by Tukey's post hoc test. Each experiment was repeated three times