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Fig. 5 | Molecular Medicine

Fig. 5

From: The mechanism of nicotinamide on reducing acute lung injury by inhibiting MAPK and NF-κB signal pathway

Fig. 5

The effect of NAM on cell viability and pro-inflammatory related genes in RAW264.7. a The effect of NAM on cell viability. The effect of NAM on cell activity was detected by CCK. The concentrations of NAM were 50 μM, 100 μM, 200 μM, 500 μM, 1 mM, 2 mM, 5 mM, 10 mM and 20 mM respectively. bd The gene levels of IL-6, IL-1β and TNF-α were detected using qRT-PCR in raw264.7 and normalized to that of β-actin. The values are presented as the mean ± SD (*p < 0.05, **p < 0.001, ***p < 0.001 and ****p < 0.0001)

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