Fig. 7
Representative images evaluating mTNF-α expression in human IBD specimens and linear regression analysis. A Targeted US imaging and immunofluorescence results (green fluorescence) demonstrated the weakest mTNF-α expression in normal intestines and the strongest mTNF-α expression in severe lesions compared with mild lesions. From left to right: gross specimens, B-mode US images, CEUS images after injection of MBcon, CEUS images after injection of MBTNF-α, H&E staining and immunohistochemical pictures (× 200, scale bar = 100 μm). B The NID and mTNF-α expression at the molecular level had a linear correlation with a coefficient of determination of R2 = 0.6947 (P < 0.001). NID normalized intensity differences