Fig. 2

Angiotensin II-induced hypertension model. A–F change in MAP, SBP, DBP, heart rate, body temperature, and activity levels during angiotensin II infusion. Dark cycle and light cycle average values were collected prior to and during angiotensin II infusion. Osmotic pumps were implanted on day 0. Data is collected continuously and averaged over the 12 h light and dark cycles separately on each day. Day 0 values represent an average of 3 days prior to implantation of osmotic pumps delivering angiotensin II. Dashed lines represent data in normotensive animals in light and dark cycles. A MAP, B SBP and C DBP significantly increase during angiotensin II infusion. Data are represented as mean ± SEM, n = 41–50, ****p < 0.0001, light cycle vs. dark cycle, ^####p < 0.0001, over time, two-way ANOVA with Sidak’s multiple comparison test. D HR decreases during angiotensin II infusion. Dark cyle HR remains significantly elevated compared to light cycle HR. Data are represented as mean ± SEM, n = 41–50, ****p < 0.0001, light cycle vs. dark cycle, ^####p < 0.0001, over time, two-way ANOVA with Sidak’s multiple comparison test. E body temperature decreases during angiotensin II infusion. Dark cycle body temperature remains significantly elevated compared to light cycle body temperature. Data are represented as mean ± SEM, n = 41–50, ****p < 0.0001, light cycle vs. dark cycle, ^####p < 0.0001, over time, two-way ANOVA with Sidak’s multiple comparison test. F activity levels decrease during angiotensin II infusion, with dark cycle activity levels remaining significantly elevated compared to light cycle activity levels. Data are represented as mean ± SEM, n = 41–50, ****p < 0.0001, light cycle vs. dark cycle, ^####p < 0.0001, over time, two-way ANOVA with Sidak’s multiple comparison test