Fig. 4

miR-124-3p is one of the targets of MAPKAPK5-AS1. A Bioinformatics revealed the common binding sites between MAPKAPK5-AS1 and miR-124-3p. B Expression of miR-124-3p in H9C2 cells transfected by miR-NC or miR-124-3p mimic. C Relative luciferase activity for cells transfected with miR-NC, miR-124-3p mimic with MAPKAPK5-AS1 WT or MAPKAPK5-AS1 MUT. D RNA pull-down for the fold enrichment of MAPKAPK5-AS1 in cells transfected with Bio-NC, Bio-miR-124-3p or Bio-miR-124-3p MUT. E Expression of miR-124-3p in H9C2 cells transfected by si-NC, or si-MAPKAPK5-AS1. F MAPKAPK5-AS1 expression in H9C2 cells transfected by miR-NC or miR-124-3p mimic. Each column represents the mean ± SEM. Statistical differences were evaluated with Student’s t-test or One-way ANOVA followed by Tukey’s post-hoc test. *P < 0.05, **P < 0.01, ***P < 0.001, n = 3