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Fig. 5 | Molecular Medicine

Fig. 5

From: Long noncoding RNA MAPKAPK5-AS1 promoted lipopolysaccharide-induced inflammatory damage in the myocardium by sponging microRNA-124-3p/E2F3

Fig. 5

miR-124-3p mediates the effects of MAPKAPK5-AS1 on the apoptosis and inflammatory response of H9C2 cells induced by LPS. A MAPKAPK5-AS1 expression in H9C2 cells transfected with vector or pc-MAPKAPK5-AS1. B Cell flow cytometry for cells treated by vehicle, LPS + pc-MAPKAPK5-AS1 or LPS + pc-. MAPKAPK5-AS1 + miR-124-3p mimic. C Western blot for protein expressions of t-casp3, c-casp3, t-PARP, e-PARP, Bax, Bcl2, and GAPDH in cells treated by vehicle, LPS, LPS + pc-MAPKAPK5-AS1 or LPS + pc-MAPKAPK5-AS1 + miR-124-3p mimic. D Western blot for protein expression levels of IL-1β, MCP-1, IL-6, TNF-α, MMP2, and MMP9 in cells treated by vehicle, LPS, LPS + pc-MAPKAPK5-AS1 or LPS + pc-MAPKAPK5-AS1 + miR-124-3p mimic. Each column represents the mean ± SEM. Statistical differences were evaluated with Student’s t-test or One-way ANOVA followed by Tukey’s post-hoc test. *P < 0.05, **P < 0.01, n = 3

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Molecular Medicine

ISSN: 1528-3658